Electropulsation as an alternative method for protein extraction from yeast

Electropulsation as an alternative method for protein extraction from yeast.

The application of series of high intensity electric pulses to a yeast suspension provoked a considerable release of some cytoplasmic proteins, glutathione reductase, 3-phosphoglycerate kinase and alcohol dehydrogenase. A maximal yield was achieved 3-8 h after pulsation. The electro-induced protein efflux was accelerated by pretreatment with the reducing agent dithiothreitol and showed a strong...

Extraction and evaluation of gelatin from yellow fin tuna (Thunnus albacares) skin and prospect as an alternative to mammalian gelatin

One of the mainly popular consumed colloid protein materials in pharmaceutics, medical, food and military industries is Gelatin. Especially from warm-water fish gelatin report poss similar characteristics to mammalian’s gelatin .Yellow fin tuna (Thunnus albacares) gelatin skin, lots of waste in form of skin and bones of the fish are produced every day. Analysis factors were extracted alkaline g...

Rapid and reliable protein extraction from yeast.

The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Sacc...

Somatic Embryogenesis: An Alternative Method for in vitro Micropropagation

Plant tissue culture is an alternative method of commercial propagation and is being used widely for the commercial propagation of a large number of plant species, including many medicinal plants. Somatic embryogenesis is a process by which asexual or somatic cells are induced to form embryos in culture. Somatic embryogenesis is a multi-step regeneration process starting with formation of pro-e...

Hydrodynamic Cavitation: An Alternative Method for Algae Removal from Water Resources